The Biuret test

LC5.3.2.1

The Biuret test

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Description

In this experiment the students get to know a chemical detection for proteins. For this purpose, different samples are mixed with Biuret solution. The students realise that a positive detection is indicated by a violet colouring of the sample. This is caused by a complex formation of the copper ions with the peptide bonds.

Components of equipment sets

1	6726710 4	Potassium sodium tartrate, 250 g
1	6729600 4	Copper(II) sulfate-5-hydrate, 100 g Danger H302 H318 H400 H410
1	6738400 4	Sodium hydroxide solution, diluted, aprox. 2 M, 500 ml Danger H314 H290
1	6735700 4	Sodium chloride, 250 g
1	6753410 4	Water, pure, 5 l
1	30945 4	Universal pencil
2	6659531 4	Dropping pipette 150 mm x 7 mm Ø
2	6659541 8	Dropper bulb
1	6045682 4	Powder spatula, steel, 185 mm
1	667057 4	Test tube rack metal, 20 mm Ø
3	6640421 4	Test tube Fiolax 16 mm x 160 mm
3	667253 4	Rubber stopper solid, 14...18 mm Ø
1	610010 8	Laboratory safety goggles
1	667609 4	Safety gloves, nitrile rubber, size 8
1	6738400 4	Sodium hydroxide solution, diluted, aprox. 2 M, 500 ml Danger H314 H290

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The Biuret test

Description

Components of equipment sets

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