The isoelectric point of amino acids

LC5.3.1.5

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Description

In this experiment, the students learn about the isoelectric point as a characteristic of amino acids. They identify the isoelectric point through precipitation of the amino acid at the pH value corresponding to the isoelectric point. In doing this, they find out that amino acids as ampholytes can react both basic and acidic depending on the pH value. They also discuss the relevance of the isoelectric point for protein electrophoresis.

Components of equipment sets

1	665212 4	Glass stirring rod 200 x 8 mm Ø
2	6659541 8	Dropper bulb
2	6659531 4	Dropping pipette 150 mm x 7 mm Ø
1	6045682 4	Powder spatula, steel, 185 mm
1	MA90204 8	Universal indicator paper pH 1...14, roll
1	602022 4	Beaker Boro 3.3, 100 ml, squat
1	610010 8	Laboratory safety goggles
1	667609 4	Safety gloves, nitrile rubber, size 8
1	6738420 4	Sodium hydroxide solution, 1 mol/l, 500 ml Danger H314 H290
1	6746900 4	Hydrochloric acid, 1 mol/l, 500 ml Warning H290
1	6752530 4	L(-)-Tyrosine, 25 g
1	6753410 4	Water, pure, 5 l

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The isoelectric point of amino acids

Description

Components of equipment sets

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