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Urea splitting by urease and its inhibition - Digital

Urea splitting by urease and its inhibition - Digital

LB4.2.3.2C

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Description

In this experiment the students will study the enzymatic splitting of urea by urease. Since ammonium ions and carbonate ions are produced during the cleavage, the course of the reaction and thus the activity of the enzyme can be followed via the increase in conductivity. In addition, the students recognise that the enzyme urease (representative of the class of enzymes) can be inhibited by heavy metals.

Components of equipment sets

1	6721700 4	Urea, 100 g
1	6752800 4	Urease (1 U/mg), 1 g
2	30121 8	Stand base MF
2	301271 4	Stand rod 40 cm, 10 mm Ø
1	30109 4	Bosshead S
1	666555 4	Universal clamp 0...80 mm
1	6045682 4	Powder spatula, steel, 185 mm
1	30945 4	Universal pencil
1	665754 4	Measuring cylinder 100 ml, with plastic base
2	602022 4	Beaker Boro 3.3, 100 ml, squat
1	664131 4	Beaker Boro 3.3, 400 ml, squat
1	524005W2	Mobile-CASSY 2 WiFi
1	5240671 4	Conductivity adapter S
1	529670 4	Conductivity sensor
1	607105 8	Magnetic stirrer mini
1	666851 4	Stirring magnet, 25 mm x 6 mm Ø, circular
1	610010 8	Laboratory safety goggles
1	6729600 4	Copper(II) sulfate-5-hydrate, 100 g Danger H302 H318 H400 H410
1	6753410 4	Water, pure, 5 l

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Urea splitting by urease and its inhibition - Digital

Description

Components of equipment sets

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