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Separation of a leaf extract with column chromatography

Separation of a leaf extract with column chromatography

VC2.4.4.1

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Description

The process of chromatography was developed by Tsvet on leaf pigments at the beginning of the 20th century. Although ridiculed at first, the technique has since become one the most important methods in organic chemistry. In experiment C2.4.4.1, a leaf extract is produced and separated into its components by means of column chromatography.

Components of equipment sets

1	665592	Chromatography column, 235 x 20 mm diam.
1	665073	Dropping funnel, 75 ml, ST 29
2	664101 4	Beaker, DURAN, 100 ml, squat
1	602012 8	Beaker, Boro 3.3, 600 ml, tall
1	667092	Mortar 30 ml, 56 mm x 36 mm
1	667091	Pestle 88 mm
1	665005	Funnel, Boro 3.3, 100 mm diam.
1	661038	Round filter, type 615, 150 mm Ø, Set of 100
1	661161 4	Narrow-neck glass bottle, amber glass, 100 ml
1	602347	Laboratory bottle, 500 ml, GL 45 thread
1	665754	Measuring cylinder 100 ml, with plastic base
1	665756	Measuring cylinder 500 ml, with plastic base
1	665217 4	Glass stirring rod, 500 mm x 8 mm diam., set of 10
1	ADAHCB602H	Precision Balance, 600 g /0,01g, with USB interface
1	665025	Powder funnel, 100 mm diam., plastic
1	6610771	Warning labels, GHS
1	667050 4	Test tube rack, plastic, for 9 tubes, 18 mm Ø
1	664043	Test tubes, Fiolax, 16 x 160 mm, set of 10
9	667253	Rubber stopper solid, 14...18 mm Ø
1	666966	Spoon-ended spatula polyamid, 180 mm
1	665953	Dropping pipette 150 mm x 7 mm Ø, 10 pieces
1	666584 4	Filtration stand for two funnels
1	665954	Dropper bulb, 10 pieces
1	30002	Stand base, V-shaped, small
1	30042	Stand rod, 47 cm, 12 mm diam.
2	30109	Bosshead S
2	666555	Universal clamp 0...80 mm
1	6708200 4	Petroleum ether, 90...110 °C, 250 ml Danger H225 H304 H315 H336 H411
1	6721000 4	Glass wool, 10 g
1	6748210	Sea sand, purified, 1 kg
1	6700410 4	Acetone, 1 l
1	6610581	Silica gel, 35-70 mesh, 1 kg
1	6610821	Stopcock grease
1		additionally required: green leaves, dry or fresh

Experiment Instructions

PDF (Experiment description)

C2.4.4.1 Separation of a leaf extract with column chromatography

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Separation of a leaf extract with column chromatography

Description

Components of equipment sets

Experiment Instructions

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