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Separation of a leaf extract with column chromatography

Separation of a leaf extract with column chromatography

VC2.4.4.1

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Description

The process of chromatography was developed by Tsvet on leaf pigments at the beginning of the 20th century. Although ridiculed at first, the technique has since become one the most important methods in organic chemistry. In experiment C2.4.4.1, a leaf extract is produced and separated into its components by means of column chromatography.

Components of equipment sets

1	665592 4	Chromatography column, 235 x 20 mm diam.
1	665073	Dropping funnel, 75 ml, ST 29
2	664101 4	Beaker, DURAN, 100 ml, squat
1	602012 8	Beaker, Boro 3.3, 600 ml, tall
1	667092 4	Mortar 30 ml, 56 mm x 36 mm
1	667091 4	Pestle 88 mm
1	665005 4	Funnel, Boro 3.3, 100 mm diam.
1	661038 4	Round filter, Type 595, 150 mm diam., 100 pcs.
1	661161 4	Narrow-neck glass bottle, amber glass, 100 ml
1	602347 4	Laboratory bottle, 500 ml, GL 45 thread
1	665754 4	Measuring cylinder 100 ml, with plastic base
1	665756 4	Measuring cylinder 500 ml, with plastic base
1	665217 4	Glass stirring rod, 500 mm x 8 mm diam., set of 10
1	ADAHCB602H	Precision Balance, 600 g /0,01g, with USB interface
1	665025 4	Powder funnel, 100 mm diam., plastic
1	6610771 8	Warning labels, GHS
1	667050 4	Test tube rack, plastic, for 9 tubes, 18 mm Ø
1	664043 4	Test tubes, Fiolax, 16 x 160 mm, set of 10
9	667253 4	Rubber stopper solid, 14...18 mm Ø
1	666966 4	Spoon-ended spatula polyamid, 180 mm
1	665953 8	Dropping pipette 150 mm x 7 mm Ø, 10 pieces
1	666584 4	Filtration stand for two funnels
1	665954 4	Dropper bulb, 10 pieces
1	30002 4	Stand base, V-shaped, small
1	30042 4	Stand rod, 47 cm, 12 mm diam.
2	30109 4	Bosshead S
2	666555 4	Universal clamp 0...80 mm
1	6708200 4	Petroleum ether, 90...110 °C, 250 ml Danger H225 H304 H315 H336 H411
1	6721000 4	Glass wool, 10 g
1	6748210 4	Sea sand, purified, 1 kg
1	6700410 4	Acetone, 1 l Danger H225 H319 EUH066 H336
1	6610581	Silica gel, 35-70 mesh, 1 kg
1	6610821	Stopcock grease
1		additionally required: green leaves, dry or fresh

Experiment Instructions

PDF (Experiment description)

C2.4.4.1 Separation of a leaf extract with column chromatography

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Separation of a leaf extract with column chromatography

Description

Components of equipment sets

Experiment Instructions

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