Separation of a leaf extract with column chromatography

VC2.4.4.1
Separation of a leaf extract with column chromatography
Separation of a leaf extract with column chromatography

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Description

The process of chromatography was developed by Tsvet on leaf pigments at the beginning of the 20th century. Although ridiculed at first, the technique has since become one the most important methods in organic chemistry. In experiment C2.4.4.1, a leaf extract is produced and separated into its components by means of column chromatography.

Components of equipment sets

1 665592  4  Chromatography column, 235 x 20 mm diam. LD
1 665073 Dropping funnel, 75 ml, ST 29 LD
2 664101  4  Beaker, DURAN, 100 ml, squat LD
1 602012  8  Beaker, Boro 3.3, 600 ml, tall LD
1 667092  4  Mortar 30 ml, 56 mm x 36 mm LD
1 667091  4  Pestle 88 mm LD
1 665005  4  Funnel, Boro 3.3, 100 mm diam. LD
1 661038  4  Round filter, Type 595, 150 mm diam., 100 pcs. LD
1 661161  4  Narrow-neck glass bottle, amber glass, 100 ml LD
1 602347  4  Laboratory bottle, 500 ml, GL 45 thread LD
1 665754  4  Measuring cylinder 100 ml, with plastic base LD
1 665756  4  Measuring cylinder 500 ml, with plastic base LD
1 665217  4  Glass stirring rod, 500 mm x 8 mm diam., set of 10 LD
1 ADAHCB602H Precision Balance, 600 g /0,01g, with USB interface LD
1 665025  4  Powder funnel, 100 mm diam., plastic LD
1 6610771  8  Warning labels, GHS LD
1 667050  4  Test tube rack, plastic, for 9 tubes, 18 mm Ø LD
1 664043  4  Test tubes, Fiolax, 16 x 160 mm, set of 10 LD
9 667253  4  Rubber stopper solid, 14...18 mm Ø LD
1 666966  4  Spoon-ended spatula polyamid, 180 mm LD
1 665953  8  Dropping pipette 150 mm x 7 mm Ø, 10 pieces LD
1 666584  4  Filtration stand for two funnels LD
1 665954  4  Dropper bulb, 10 pieces LD
1 30002  4  Stand base, V-shaped, small LD
1 30042  4  Stand rod, 47 cm, 12 mm diam. LD
2 30109  4  Bosshead S LD
2 666555  4  Universal clamp 0...80 mm LD
1 6708200  4  Petroleum ether, 90...110 °C, 250 ml LD
GHS02GHS07GHS08GHS09 Danger H225 H304 H315 H336 H411
1 6721000  4  Glass wool, 10 g LD
1 6748210  4  Sea sand, purified, 1 kg LD
1 6700410  4  Acetone, 1 l LD
GHS02GHS07 Danger H225 H319 EUH066 H336
1 6610581 Silica gel, 35-70 mesh, 1 kg LD
1 6610821 Stopcock grease LD
1 additionally required:
green leaves, dry or fresh

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C2.4.4.1 Separation of a leaf extract with column chromatography
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