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Enzymatics: Splitting urea with urease

VC6.1.2.1
Enzymatics: Splitting urea with urease
Enzymatics: Splitting urea with urease
Enzymatics: Splitting urea with urease

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Description

Experiment C6.1.2.1 takes a closer look at the enzyme urease. Urease splits urea into ammonia and carbonate ions. Since the urea solution does not conduct electricity, but a solution with ammonia and carbonate does, the course of the reaction can be recorded with conductivity measurements. The maximum rate of reaction and the reaction order are determined. Also an inhibitor is tested.

Components of equipment sets

1 524005W2 Mobile-CASSY 2 WiFi LD
1 524220  4  CASSY Lab 2 LD
1 5240671  4  Conductivity adapter S LD
1 529670  4  Conductivity sensor LD
1 607105  8  Magnetic stirrer mini LD
1 30011  4  Saddle base LD
1 30126  4  Stand rod 25 cm, 10 mm Ø LD
1 30109  4  Bosshead S LD
1 666555  4  Universal clamp 0...80 mm LD
2 602010  4  Beaker, Boro 3.3, 150 ml, tall LD
1 665997  4  Graduated pipette 10 ml LD
1 666003  4  Pipetting ball (Peleus ball) LD
1 665793  4  Volumetric flask, Boro 3.3, 100 ml LD
2 664153  4  Watch glass dish 60 mm Ø LD
2 604170  4  Powder funnel d=65mm LD
1 664043  4  Test tubes, Fiolax, 16 x 160 mm, set of 10 LD
2 667253  4  Rubber stopper solid, 14...18 mm Ø LD
1 667050  4  Test tube rack, plastic, for 9 tubes, 18 mm Ø LD
1 666961  4  Double-ended microspatula, stainless steel, 185 mm LD
1 ADAHCB602H Precision Balance, 600 g /0,01g, with USB interface LD
1 6703900  4  Ammonium carbonate, 100 g LD
GHS07 Warning H302
1 6721700  4  Urea, 100 g LD
1 6752810  4  Urease (1 U/mg), 5 g LD
1 6729600  4  Copper(II) sulfate-5-hydrate, 100 g LD
GHS09GHS05GHS07 Danger H302 H318 H400 H410
1 6753400  4  Water, pure, 1 l LD
1 additionally required: PC with Windows 7 or higher with WIFI or USB connection

Experiment Instructions

PDF (Experiment description) PDF (Experiment description) C6.1.2.1 Enzymatics: Splitting urea with urease
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Physics
Chemistry
Biology
Technology

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